Section

School of Biological Sciences

Location:

Highfield Campus

Salary

£31,236 to £35,608 per annum

Part Time Fixed Term until 31/05/2027 (17.5 hours per week)

Closing Date:

Wednesday 24 September 2025

Interview Date

To be confirmed

Reference

3210225BJ

The Glycoprotein Therapeutics Laboratory, led by Professor Max Crispin, focuses on developing antiviral vaccines and antibody-based therapies against cancer. A key area of interest is HIV vaccine design, particularly understanding how glycans influence viral protein structure and function, and how they can be manipulated for therapeutic applications.

We are seeking a part-time Research Technician to support laboratory research on HIV-1 glycobiology. The role includes mammalian cell culture, glycoprotein and pseudovirus production, protein purification, and sample preparation for mass spectrometry. The postholder will ensure smooth day-to-day running of experiments, accurate data recording, and high-quality outputs, while contributing to lab maintenance and collaborative projects.

Key Responsibilities

• Mammalian Cell Culture: Maintain mammalian cell lines used for glycoprotein and pseudovirus production. Perform transfections and monitor cell growth to ensure optimal conditions.

• Glycoprotein Production: Express HIV-1 envelope glycoproteins in mammalian cells. Purify proteins using nickel affinity and size-exclusion chromatography. Prepare samples for mass spectrometry analysis.

• Pseudovirus Production: Assist in the generation of HIV-1 pseudoviruses. Conduct quality checks to ensure reproducibility and consistency across batches.

• Laboratory Support: Prepare buffers, reagents, and culture media as needed. Maintain laboratory equipment and ensure compliance with safety standards. Support adherence to protocols and maintain accurate records.

• Data Management: Document procedures, sample inventories, and experimental results. Keep detailed logs for pseudovirus and glycoprotein samples.

• Collaboration: Work closely with postdoctoral researchers, PhD students, and technical staff. Provide technical support across HIV-1 glycobiology projects.