Practical Microscopy for Technicians
Learn about microscope components, configurations, resolution, and magnification, including brightfield and phase contrast techniques, with hands-on practice time.
Type of Course: Practical Skills
Course Reference: HFFC-003
Course duration: 1 day
Hosting Mode: Onsite only
This course is brought to you by HEaTED, with support of the Royal Microscopical Society.
Learn about microscope components, configurations and light paths
Learn about resolution and magnification
Gain an overview of brightfield and its components
Find out about the Köhler illumination set-up alignment process
Receive an introduction to the phase contrast technique and phase contrast components
Benefit from hands-on practice time with the microscope for brightfield and phase contrast.
Find out more:
Outcomes
Understand about microscope components, configurations and light paths
Understand about resolution and magnification and how to use the correct setups and why
Be able to set up a brightfield microscope and its components
Be able to perform the Köhler illumination set-up alignment process
Understand when and how to use the phase contrast technique and phase contrast components
Session Content
History, Microscope Anatomy, Magnification, Resolution, Diffraction & Köhler (Lecture)
Köhler (Practical)
Know your Lens: Aberration, Lens Design, Working Distance, Refractive Index Matching & Nomenclatures/Labels (Lecture)
Microscope Maintenance & Cleaning (Lecture/Practical)
Contrast Techniques: Phase Contrast & Dark Field, DIC & Polarisation, Epifluorescence basics (Lecture)
Phase Contrast, DIC and Polarisation (Practical)
Lenses, Open Optical Bench, NA & Swinging Apertometer (Practical)
Flexible session; this may run subject to remaining time available and may include:
Epifluorescence microscopy (Practical)
Cameras, Image Capture, considerations for image analysis: (Pixels, Chip Type, Resolution, Signal to Noise, Dynamic Range & Scaling) (Lecture)
Course Fee
HEaTED members
Onsite: £322.00
Course credits
2 x online course credits or
1 x onsite course credits
Non-members
Onsite: £519.95
Notes
This activity is subject to minimum group numbers. For HEaTED members, we offer one free place for every 10 bookings, and two for groups of 15 or above.